Beilstein J. Org. Chem.2013,9, 1768–1773, doi:10.3762/bjoc.9.205
, dimethyl furan-2,4-dicarboxylate. The crypticmetabolite represents the first non-azinomycin related compound to be isolated and characterized from the soil bacterium, S. sahachiroi. The results from this study suggest that abolishing production of otherwise predominant natural products through genetic
knockout may constitute a means to “activate” the production of novel secondary metabolites that would otherwise lay dormant within microbial genome sequences.
Keywords: crypticmetabolite; dimethyl furan-2,4-dicarboxylate; genetic knockout; natural products; non-ribosomal peptide synthetase module
overproduction of the crypticmetabolite dimethyl furan-2,4-dicarboxylate and led to complete abolishment of azinomycin production. It is the first non-azinomycin related metabolite to be reported from the S. sahachiroi strain, a microorganism that has been mined for its natural product constituents since 1954
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Graphical Abstract
Figure 1:
Schematic diagram illustrating the phenotypic effects arising through deletion of the aziA2 gene.